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KMID : 0603520010060040222
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Journal of Korean Association of Cancer Prevention
2001 Volume.6 No. 4 p.222 ~ p.231
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p53 Mutation in Hepatocellular Carcinomas
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Choi Won-Hee
Lee Sang-Lak
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Abstract
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Background: Hepatocellular carcinoma (HCC)is one of the most common primary malignant tumor of the liver in Africa and Asia. The p53 gene has been found in association with many human cancers including HCC. The prevalence and features of p53 mutations in HCC vary significantly between different geographic areas, may be due to different causing factors. An analysis of p53 gene mutation by PCR and direct DNA sequencing method is most accurate but time consuming difficult process so that only one study with 13 HCC patients has been reported in Korean literature.
Methods: The status of exons 5,6,7,8 of the p53 gene was analyzed in 20 HCC tumor tissues and 20 non-tumor bearing liver tissues from 20 HCC patients using PCR and direct DNA sequencing. DNA was separated and extracted from formalin fixed paraffin embedded tissues.
Results Abnormalities of the p53 gene were found in six out of 20 HCC but none from 20 non-tumor bearing tissues. By direct DNA sequencing, mutations of the p53 gene were found in 5 cases among 6 PCR amplified products. All were G deletions in exon 7 (14,073 bp) on the 249th codon with frame shift. In one case, the site of mutation was not found on the examined exon 5-8
Conclusion: Although total number of observation was small, but the characteristic feature, location of p53 gene mutation occurred in Korean HCC shown in this study may be utilized importantly in the diagnosis and treatment of HCC in Korea when the number of study will expanded.
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KEYWORD
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Hepatocellular carcinoma, PCR, Direct DNA sequencing, p53 gene mutation
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